Journal: British Journal of Cancer

Article Title: microRNA-22, downregulated in hepatocellular carcinoma and correlated with prognosis, suppresses cell proliferation and tumourigenicity

doi: 10.1038/sj.bjc.6605895

Figure Lengend Snippet: Endogenous miR-22 expression level in human normal liver. Data are shown as miRNA copies per 20 pg total RNA (equivalent of approximately one cell).

Article Snippet: For the gain-of-function study, miR-22 mimics (double-stranded RNA oligonucleotides) and scrambled control RNA were synthesised by GenePharma.

Techniques: Expressing

Journal: British Journal of Cancer

Article Title: microRNA-22, downregulated in hepatocellular carcinoma and correlated with prognosis, suppresses cell proliferation and tumourigenicity

doi: 10.1038/sj.bjc.6605895

Figure Lengend Snippet: miR-22 suppresses cell proliferation and tumourigenicity. ( A ) miR-22 expression in HCC cell lines Hep3B and SMMC7721, and transfected with control (ctrl) RNA or miR-22 mimics for 4 days. ( B ) Cell proliferation of these cells transfected as in A was measured in the indicated time periods using MTT assay. ( C ) Effect of miR-22 on tumourigenicity in nude mouse xenograft model. The transfected Hep3B and SMMC7721 cells were injected subcutaneously into either side of the posterior flank of the same nude mice respectively. Tumour growth curve is shown as indicated. Data are shown as mean±s.d. ( n =6). ** P <0.01.

Article Snippet: For the gain-of-function study, miR-22 mimics (double-stranded RNA oligonucleotides) and scrambled control RNA were synthesised by GenePharma.

Techniques: Expressing, Transfection, Control, MTT Assay, Injection

Journal: British Journal of Cancer

Article Title: microRNA-22, downregulated in hepatocellular carcinoma and correlated with prognosis, suppresses cell proliferation and tumourigenicity

doi: 10.1038/sj.bjc.6605895

Figure Lengend Snippet: miR-22 targets human HDAC4. ( A ) Sequence alignment of miR-22 and its conserved target site in HDAC4 3′ UTR (downloaded from TargetScan). ( B ) Luciferase expression of the indicated construct co-transfected with control RNA or miR-22 mimics. Data are shown as mean±s.d. ( n =6). ** P <0.01. ( C ) Hep3B and SMMC7721 cells were transfected as in . After 48 h, human HDAC4 and β -actin were detected by western blot.

Article Snippet: For the gain-of-function study, miR-22 mimics (double-stranded RNA oligonucleotides) and scrambled control RNA were synthesised by GenePharma.

Techniques: Sequencing, Luciferase, Expressing, Construct, Transfection, Control, Western Blot

Journal: British Journal of Cancer

Article Title: microRNA-22, downregulated in hepatocellular carcinoma and correlated with prognosis, suppresses cell proliferation and tumourigenicity

doi: 10.1038/sj.bjc.6605895

Figure Lengend Snippet: Inhibition of HDAC4 suppresses cell proliferation. Hep3B and SMMC7721 cells were transfected with control RNA or HDAC4 siRNA ( A ), or treated with TSA (100 n M ) ( B ) for the indicated time periods. Cell proliferation was measured using MTT assay. Data are shown as mean±s.d. ( n =6). ** P <0.01.

Article Snippet: For the gain-of-function study, miR-22 mimics (double-stranded RNA oligonucleotides) and scrambled control RNA were synthesised by GenePharma.

Techniques: Inhibition, Transfection, Control, MTT Assay

Journal: Molecular Medicine Reports

Article Title: Downregulation of inhibitor of apoptosis-stimulating protein of p53 inhibits proliferation and promotes apoptosis of gastric cancer cells

doi: 10.3892/mmr.2015.3587

Figure Lengend Snippet: iASPP expression is significantly downregulated in gastric cancer cells following infection with iASPP-siRNA-lentivirus, while the control siRNA had no effect on iASPP protein levels. (A) iASPP expression in MKN-45 cells was inhibited following iASPP-siRNA-lentivirus infection. (B) iASPP expression in SGC-7901 cells was inhibited following iASPP-siRNA-lentivirus infection. Values are expressed as the mean ± standard deviation; * P<0.05. iASPP, inhibitor of apoptosis-stimulating protein of p53; siRNA, small interfering RNA.

Article Snippet: Downregulation of iASPP was achieved by infecting the cells with the iASPP-small interfering (si)RNA lentivirus (Genepharma Co., Ltd., Shanghai, China).

Techniques: Expressing, Infection, Standard Deviation, Small Interfering RNA

Journal: Molecular Medicine Reports

Article Title: Downregulation of inhibitor of apoptosis-stimulating protein of p53 inhibits proliferation and promotes apoptosis of gastric cancer cells

doi: 10.3892/mmr.2015.3587

Figure Lengend Snippet: Effects of iASPP expression on GC cells. (A) An MTT assay indicated that GC cell proliferation was significantly inhibited following iASPP-siRNA-lentivirus infection. (B) Inhibition of iASPP expression inhibited the colony-forming ability of GC cell lines. (C) Inhibition of iASPP promoted GC cell apoptosis. Values are expressed as the mean ± standard deviation; * P<0.05, ** P<0.01 vs. control. iASPP, inhibitor of apoptosis-stimulating protein of p53; siRNA, small interfering RNA; GC, gastric cancer; PE, phycoerythrin; UL, upper left; UR, upper right; LL, lower left; LR, lower right.

Article Snippet: Downregulation of iASPP was achieved by infecting the cells with the iASPP-small interfering (si)RNA lentivirus (Genepharma Co., Ltd., Shanghai, China).

Techniques: Expressing, MTT Assay, Infection, Inhibition, Standard Deviation, Small Interfering RNA